| Founder's Educations and Experiences: |
| SUMMARY OF QUALIFICATIONS: An accomplished Ph.D. with solid knowledge in both organic chemistry and biotechnology. Over 10 years of cGLP and cGMP experience. More than 10 years experiences in assay development, high throughput screening, enzyme engineering and biocatalysis. Experienced in converting laboratory innovations into products. EXPERIENCE and ACHIEVEMENTS: Founder and President of Sunmeck Inc. (www.sunmeck.com) and (www.sunmeck.net) (2003-Aug. 2006) · Consulting consulting for Pharmaceutical and related industries for the followings: Current Good Manufacture Practice (CGMP) compliant, Quality system improvement, · Regulatory Document preparation and filing. Director, Technology Development, ChemPacific (www.chempacific.com) (2002) Senior Research Scientist at Chiragene/Cambrex (www.cambrex.com) (1998-2001) Research Scientist at Celgene (www.celgene.com) (1996-1997) · Recipient of John F. White Technical Achievement Award, 2000, Cambrex Corporation for dramatically improving several industrial enzymes. · Independently and successfully took charge of Celgene's enzyme tailoring technology. · Developed high throughput assays to screen enzyme libraries to obtain novel engineered enzymes used in chiral syntheses. · Screened more than 20 amino transferase libraries (about 1,000,000 clones). · has lead to several products, including one step synthesis of drug substance S-amphetamine sulfate from Phenyl acetone, filed to the FDA. Post-doctoral Fellow at Hoffmann La-Roche with Dr. Arthur Levin (www.roche.com) (1993-1996) · Set up a molecular biology laboratory from ground up. · Established a 96-well format homogenous fluorescent assay to quantify an enzyme called telomerase. · Purified telomerase by gel filtration, ion exchange, affinity columns, magnetic beads, preparative isoelectro-focusing, and native gel electrophoresis. · Developed a semi-automatic 96-well plate assay to quantify liquid droplet induction in human breast tumor cells by retinoids. · Used Differential Display to investigate gene statement patterns in different drug treated cells. Post-doctoral Fellow with Professor Sidney Pestka at UMDNJ (www.umdnj.edu) (1990-1993) · Carried out protein engineering to generate chimerical interferon receptor. · Successfully purified and renatured the human interferon gamma receptor extracellular domain. · Investigated interferon signal transduction: cell fusion; major histo-compatibility complex (MHC) antigen induction; fluorescence activated cell sorting (FACS); PCR; etc. · Invented a method of double strain plasmid DNA site-specific mutation. · U. S. Patent 5,633,157, (1997). Research assistant with Professor Masayori Inouye at UMDNJ (www.umdnj.edu) (1987-1990) · Studied enzyme engineering to make an enzyme working under desired conditions. · Discovered the important functions of pro-sequence of serine protease. · Invented a method to recover enzyme activity which had been lost by acid or heat denaturation, · U. S. Patent 5,191,063, (1993), licensed to MOCHIDA pharmaceutical Inc. (Japan). · Discovered a new type of specific inhibitor of the protease. Research Assist. Shanghai Institute of Organic Chemistry, Academy of China (www.sioc.ac.cn) (1982-1986) · Organic compound synthesis. EDUCATION: · Ph.D. Biochemistry with Prof. F. Jordan and M. Inouye, Rutgers University (www.rutgers.edu), 1990. · M.S. Organic Chemistry, Shanghai Institute of Organic Chemistry (www.sioc.ac.cn), China, 1985. · B.S. Analytical Chemistry, Yunnan University (www.ynu.edu.cn), Kunming, P. R. China, 1982. HONORS: · Recipient of John F. White Technical Achievement Award, 2000, Cambrex Corporation · Recipient of the Commemorative 225th Anniversary Diploma, Rutgers University, 1990, for Outstanding Dissertation, Academic Achievement, Leadership and All the Recommendations. · H. Martin Friedman Award, Chemistry Department, Rutgers University, 1990 for Overall Excellence in Chemistry. · Dean's dissertation Award, Rutgers University, 1990, for Outstanding Doctoral Dissertation. · Recipient of Graduate Fellowship, Rutgers University, 1986, 1987, 1988 and 1989, for Excellent Academic Achievement. PUBLICATIONS: 1. Zhu, X., Ohta, Y., Jordan, F. and Inouye, M. “Pro-sequence of Subtilisin Can Guide the Refolding of Denatured Subtilisin in an Intermolecular Process,” Nature 399, 483 (1989). 2. Cash, P.W., Zhu, X., Ohta, Y., Lackland, H., Inouye, M., Stein, S., Jordan, F. and Tous, G. I. “synthesis of the Pro-sequence of Subtilisin BPN,” Peptide Research 2, 292 (1989). 3. Zhu, X., Ohta, Y., Jordan, F. and Inouye, M. “Intermolecular Complementation of Protein Folding: Refolding of Denatured Subtilisin with the Aid of Exogenous Pro-sequence,” Annual Meeting of American Society for Microbiology May 14-18th (1989). 4. Zhu, X. Ph. D. Thesis: “Role of the Pro-sequence in the Reactivating of Denatured Protease Subtilisin,” University Press, Rutgers University, (1990). 5. Ohta, Y., Hojo, H., Aimoto, S., Tabayashi, T., Zhu, X., Jordan, F. and Inouye, M. “Pro-peptide as an Intramolecular Chaperone: Renaturation of Denatured Subtilisin E with a Synthetic Pro-peptide,” Molecular Microbiology 5, 1507 (1991) 6. Zhu, X., Tous, G. I., Stein, S., Inouye, M. and Jordan, F. “Refolding of Denatured Subtilisin with the Aid of a Synthetic Pro-peptide; Direct Demonstration that the Pro-sequence Guides the Folding of Secreted Enzymes,” Life Science Advances in Biochemistry 11, 34 (1992). 7. Lai, D., Zhu, X., Isotova, L., Mariano, T. M., Hibino, Y. and Pestka, S. “statement, Purification and Refolding of the Extracellular Domain of the Murine Gamma Interferon Receptor.” The Journal of Interferon Research 12, 218 (1992). 8. Lai, D., Zhu, X. and Pestka, S. “A Simple and Efficient Method for Site-directed Mutagenesis Using Double-stranded Plasmid DNA,” Nucleic Acids Research 21, 3977 (1993). 9. Zhu, X., Hu, Z., Jordan, F. and Inouye, M. “Covalently Trapped Folding Intermediate of Subtilisin E; Spontaneous Dimerization of Pro-Subtilisin E Ser49Cys Mutant in vivo and Its Auto processing in vitro,” Biochemistry 33, 562 (1994). 10. Hua, J., Zhu, X. and Rashidbaigi, M. “Sendai Virus Induced Cytokines from Peripheral Blood Leukocytes” The Journal of Interferon and Cytokine Research 15, S135 (1995). 11. Zhu, X., Kumar, R., Sharma, H. W., Dhingra, U., Hsiao, R. and Narayanan, R. “Detection of Cell Cycle Dependent Telomerase Activity in Tumor Cells” Proceeding of National Academe of Science U.S.A. 93 (12), 6091-5 (1996). 12. Sharma, H. W., Maltese, J., Zhu, X., Hans E. Kaiser and R. Narayanan “Telomeres, Telomerase and Cancer: Is the Magic Bullet Real?” Anticancer Res., 16, 511-5 (1996). 13. Zhu, X., Lewis, C. M., Haley, M.C., Bhatia, M. B., Pannuri, S., Kamat, S., Wu, W. and Bowen, A.R. StG. “Directed Evolution of Enzymes for Commercial Production of Chiral Amines” Exploiting Enzyme Technology for Industrial Application, San Diego, California, abstract and poster, February 20-21, 1997 14. Zhu, X. “Directed Evolution of Enzymes for Commercial Production of Chiral Intermediates” The Second Conference on Affinity Technology, Arlington, Virginia, abstract and oral presentation, September 29-30, 1997 |
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